Premium
Multi‐class, multi‐residue liquid chromatography/tandem mass spectrometry screening and confirmation methods for drug residues in milk
Author(s) -
Turnipseed Sherri B.,
Andersen Wendy C.,
Karbiwnyk Christine M.,
Madson Mark R.,
Miller Keith E.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3532
Subject(s) - chemistry , chromatography , residue (chemistry) , tandem mass spectrometry , veterinary drug , mass spectrometry , liquid chromatography–mass spectrometry , triple quadrupole mass spectrometer , analyte , cartridge , electrospray , selected reaction monitoring , biochemistry , mechanical engineering , engineering
This paper describes the development and optimization of a multi‐residue veterinary drug screening method for whole milk. The drug residues of regulatory interest in milk include β ‐lactams, sulfonamides, tetracyclines, fluoroquinolones, and macrolides. Milk samples were extracted with acetonitrile and the samples were then subjected to a clean‐up procedure using a bonded solid‐phase extraction cartridge and a molecular weight cut‐off filter. Liquid chromatography/tandem mass spectrometry (LC/MS/MS) triple quadrupole electrospray methods were developed to monitor for the drugs in milk. Since established tolerance levels are set for most of these drugs in milk, the initial screening procedure was semi‐quantitative, where samples were compared to the response of a positive control. The positive control, consisting of an extract from a portion of milk fortified with the drugs at half their allowed levels, was used to set the laboratory's minimum response criteria for unknown samples. Confirmatory analyses, with additional ion transitions for each residue, were performed on the same extracts. Published in 2008 by John Wiley & Sons, Ltd.