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Application of ion trap technology to liquid chromatography/mass spectrometry quantitation of large peptides
Author(s) -
Shipkova Petia,
Drexler Dieter M.,
Langish Robert,
Smalley James,
Salyan Mary Ellen,
Sanders Mark
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3511
Subject(s) - chemistry , ion trap , quadrupole ion trap , mass spectrometry , triple quadrupole mass spectrometer , fragmentation (computing) , hybrid mass spectrometer , selected reaction monitoring , ion , tandem mass spectrometry , analyte , analytical chemistry (journal) , chromatography , top down proteomics , quadrupole , collision induced dissociation , dissociation (chemistry) , atomic physics , organic chemistry , physics , computer science , operating system
Triple quadrupole mass spectrometers are generally considered the instrument of choice for quantitative analysis. However, for the analysis of large peptides we have encountered some cases where, as the data presented here would indicate, ion trap mass spectrometers may be a good alternative. In general, specificity and sensitivity in bioanalytical liquid chromatography/mass spectrometry (LC/MS) assays are achieved via tandem MS (MS/MS) utilizing collision‐induced dissociation (CID) while monitoring unique precursor to product ion transitions (i.e. selected reaction monitoring, SRM). Due to the difference in CID processes, triple quadrupoles and ion traps often generate significantly different fragmentation spectra of product ion species and intensities. The large peptidic analytes investigated here generated fewer fragments with higher relative abundance on the ion trap as compared to those generated on the triple quadrupole, resulting in lower limits of detection on the ion trap. Copyright © 2008 John Wiley & Sons, Ltd.