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Coupling of ion‐molecule reactions with liquid chromatography on a quadrupole ion trap mass spectrometer
Author(s) -
Pyatkivskyy Yuriy,
Ryzhov Victor
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3494
Subject(s) - chemistry , ion trap , mass spectrometry , hybrid mass spectrometer , quadrupole ion trap , phosphopeptide , selected reaction monitoring , triple quadrupole mass spectrometer , ion , analytical chemistry (journal) , quadrupole mass analyzer , chromatography , quadrupole , tandem mass spectrometry , peptide , atomic physics , organic chemistry , biochemistry , physics
We report for the first time a coupling of gas‐phase ion‐molecule reactions with chromatographic separations on a quadrupole ion trap mass spectrometer. The interface was accomplished by using a pulsed valve for the introduction of a volatile neutral into the ion trap. The pulsed valve controller is synchronized with the mass spectrometer software. The setup requires some minor modifications to the vacuum system of the commercial quadrupole ion trap but most of the modifications are external to the mass spectrometer. Two applications of this interface are described: differentiation between two phosphoglucose positional isomers and detection of a phosphopeptide in a peptide mixture. Both applications are using the reactivity of trimethoxyborate towards a phosphate moiety in the negative ion mode. The detection of phosphopeptides hinges on our findings that non‐phosphorylated peptide anions do not react with trimethoxyborate. This LC/MS detection can be easily visualized in terms of selected reaction monitoring. Copyright © 2008 John Wiley & Sons, Ltd.