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Development of a dual‐spray electrospray deposition system for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Erb William J.,
Owens Kevin G.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3492
Subject(s) - chemistry , extractive electrospray ionization , desorption electrospray ionization , electrospray , matrix assisted laser desorption electrospray ionization , electrospray ionization , mass spectrometry , sample preparation in mass spectrometry , chromatography , analytical chemistry (journal) , ambient ionization , desorption , ionization , matrix assisted laser desorption/ionization , thermal ionization mass spectrometry , chemical ionization , organic chemistry , ion , adsorption
A new method of matrix‐assisted laser desorption/ionization (MALDI) sample preparation using a dual‐spray electrospray deposition system is demonstrated and employed for the investigation of gas‐phase cationization reactions in the MALDI plume. The dual‐spray electrospray system is found to increase the homogeneity of the sample similarly to that of a conventional single‐spray electrospray system. The dual‐spray electrospray system allows for intimate mixing of separately prepared sample components and results in improved quantitative results. The development of this device also leads to the possibility of mixing sample components prepared in different solvents without the need to be concerned with solvent miscibility. Copyright © 2008 John Wiley & Sons, Ltd.