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Facile sequencing of oligosaccharides by matrix‐assisted laser desorption/ionisation on a hybrid quadrupole orthogonal acceleration time‐of‐flight mass spectrometer
Author(s) -
Hanrahan Sarah,
Charlwood Joanne,
Tyldesley Richard,
Langridge James,
Bordoli Robert,
Bateman Robert,
Camilleri Patrick
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.349
Subject(s) - chemistry , mass spectrometry , fragmentation (computing) , analytical chemistry (journal) , glycan , quadrupole mass analyzer , matrix assisted laser desorption/ionization , ion , oligosaccharide , chromatography , quadrupole , glycosidic bond , desorption , glycoprotein , organic chemistry , operating system , biochemistry , adsorption , computer science , physics , atomic physics , enzyme
N‐Linked oligosaccharide mixtures released from a number of standard glycoproteins were derivatised with 3‐acetylamino‐6‐acetylaminoacridine (AA‐Ac) using reductive amination. Analysis of these mixtures using an experimental matrix‐assisted laser desorption/ionisation (MALDI) hybrid quadrupole orthogonal acceleration time‐of‐flight (Q‐TOF) mass spectrometer provided detailed information about the mass distribution of the glycan derivatives. Collision‐induced dissociation of the singly protonated [M + H] + ions also gave rise to a number of product ions produced by the sequential cleavage of the glycosidic linkages. As fragmentation of the positively charged species occurred predominantly in one direction, i.e., from the non‐reducing end of the glycan to the AA‐Ac moiety, a considerable amount of information could be obtained with ease about the sequence in which the sugar residues were attached to one another. This derivatisation procedure and mass spectrometric methodology were applied successfully to neutral and acidic glycans released from proteins separated by gel electrophoresis. Copyright © 2001 John Wiley & Sons, Ltd.