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Negative ion mode matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometric analysis of oligosaccharides using halide adducts and 9‐aminoacridine matrix
Author(s) -
Becher Jana,
Muck Alexander,
Mithöfer Axel,
Svatoš Aleš,
Boland Wilhelm
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3489
Subject(s) - chemistry , mass spectrometry , adduct , iodide , desorption , analytical chemistry (journal) , ion , matrix (chemical analysis) , halide , sodium iodide , chromatography , inorganic chemistry , organic chemistry , adsorption
9‐Aminoacridine was established as a matrix for the detection of neutral oligosaccharides in negative ion mode matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF) mass spectrometry. Sodium iodide proved to be a useful additive inducing formation of stable iodide adducts of the analytes, in particular for oligosaccharides with a degree of polymerisation (DP) of three and higher. Lower oligomers (DP <3) and monosaccharides show more stable adducts with chloride ions. After optimisation of the sample preparation procedure, limits of quantitation were determined for α ‐cyclodextrin and cellopentaose at 7 and 13 pmol, respectively, with a linear detector response over two concentration orders. The iodide additive could be successfully employed on MALDI‐TOF mass spectrometers with vacuum and atmospheric pressure ion sources. The value of the new method to solve biological problems has been demonstrated by the analysis of a mixture of β ‐glucane elicitors isolated from the cell walls of Phytophthora soja e. Copyright © 2008 John Wiley & Sons, Ltd.