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Sensitive detection of phosphopeptides by matrix‐assisted laser desorption/ionization mass spectrometry: use of alkylphosphonic acids as matrix additives
Author(s) -
Kuyama Hiroki,
Sonomura Kazuhiro,
Nishimura Osamu
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3482
Subject(s) - chemistry , mass spectrometry , matrix assisted laser desorption/ionization , analyte , phosphopeptide , chromatography , matrix (chemical analysis) , desorption , adduct , maldi imaging , organic chemistry , adsorption , phosphorylation , biochemistry
Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) has been one of the most powerful tools for analyzing protein phosphorylation. However, it is frequently difficult to detect phosphopeptides with high sensitivity by MALDI‐MS. In our investigation of matrix/matrix‐additive substances for improving the phosphopeptide ion response in MALDI‐MS, we found that the addition of low‐concentration alkylphosphonic acid to the matrix/analyte solution significantly enhanced the signal of phosphopeptides. In this study, the combination of methanediphosphonic acid and 2,5‐dihydroxybenzoic acid gave the best results. In addition to enhancing the signal of the phosphopeptides, alkylphosphonic acid almost completely eliminated the signals of sodium and potassium ion adducts. We report herein sensitive detection of phosphopeptides by MALDI‐MS with the use of alkylphosphonic acids as matrix additives. Copyright © 2008 John Wiley & Sons, Ltd.