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Investigation of volatile biomarkers in liver cancer blood using solid‐phase microextraction and gas chromatography/mass spectrometry
Author(s) -
Xue Ruyi,
Dong Ling,
Zhang Si,
Deng Chunhui,
Liu Taotao,
Wang Jiyao,
Shen Xizhong
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3466
Subject(s) - solid phase microextraction , chemistry , chromatography , hexanal , mass spectrometry , gas chromatography–mass spectrometry , extraction (chemistry) , gas chromatography
Solid‐phase microextraction (SPME) followed by gas chromatography/mass spectrometry (GC/MS) was used for the detection of liver cancer volatile biomarkers. Headspace SPME conditions (fiber coating, extraction temperature and extraction time) and desorption conditions were optimized and applied to the determination of volatiles in human blood. Between the liver cancer group (n = 19) and the normal group (n = 18), positive rates of 19 volatile compounds among the total of 47 detected were found to be different with statistical significance ( p < 0.05, chi‐squared test). We suggested hexanal, 1‐octen‐3‐ol and octane, of the 19 compounds, as biomarkers of liver cancer with clinical diagnostic value. The sensitivity and specificity of 94.7% and 100% for hexanal, 84.2% and 100% for 1‐octen‐3‐ol, and 89.5% and 100% for octane were obtained, respectively, after the cutoff values had been properly established. These results show that SPME‐GC/MS is a simple, rapid and sensitive method for the investigation of volatile disease markers in human blood. Copyright © 2008 John Wiley & Sons, Ltd.