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Quantitative determination of progesterones and corticosteroids in human urine using gas chromatography/mass spectrometry: application to pelvic organ prolapse patients
Author(s) -
Shama Naz,
Bai Sang Wook,
Chung Bong Chul,
Jung Byung Hwa
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3441
Subject(s) - chemistry , chromatography , gas chromatography–mass spectrometry , detection limit , urine , mass spectrometry , selected ion monitoring , urinary system , gas chromatography , extraction (chemistry) , medicine , biochemistry
A quantitative analytical method using gas chromatography/mass spectrometry (GC/MS) to determine urinary concentrations of eight progesterones and corticosteroids has been developed. After enzymatic hydrolysis with β‐glucouronidase/arylsulfatase, urine samples were extracted by simple one‐step solid‐phase extraction. Obtained extracts were derivatized with a mixture of N ‐methyl‐ N ‐(trimethylsilyl)trifluoroacetamide/ammonium iodide/dithiothreitol and determined by GC/MS in selected ion monitoring mode to increase the sensitivity. d 4 ‐Cortisol and d 9 ‐progesterone were used as internal standards for two different steroid groups. The linear correlation coefficient was in the range of 0.9913 to 0.9998 and recoveries were over 80% for all compounds. Precision and accuracy were in the range of 0.9–18.1 and 84.1–118.7%, respectively. The limit of quantitation (LOQ) was 10 ng/mL for 11‐deoxycorticosterone and 21‐deoxycortisol and 5 ng/mL for all other analytes. The developed method was successfully applied on pelvic organ prolapsed patients (n = 10, age: 67.9 ± 4.9) and post‐menopausal (n = 10, age: 63.6 ± 5.5) control women. Urinary levels of most progesterones and corticosteroids except 11‐deoxycorticosterone decreased but only that of 17α‐hydroxyprogesterone significantly decreased in patients compared with the control groups. Thus, it is concluded that progesterones could be a factor in the pathogenesis of pelvic organ prolapse, and, among them, 17α‐hydroxyprogesterone could be a biomarker for pelvic organ prolapse. Copyright © 2008 John Wiley & Sons, Ltd.