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Trace level determination of trichloroethylene in biological samples by headspace solid‐phase microextraction gas chromatography/negative chemical ionization mass spectrometry
Author(s) -
Liu Yongzhen,
Muralidhara Srinivasa,
Bruckner James V.,
Bartlett Michael G.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3426
Subject(s) - chemistry , chromatography , mass spectrometry , solid phase microextraction , gas chromatography–mass spectrometry , gas chromatography , extraction (chemistry) , chemical ionization , detection limit , trichloroethylene , solid phase extraction , analytical chemistry (journal) , ionization , environmental chemistry , ion , organic chemistry
Abstract A gas chromatography/negative chemical ionization mass spectrometry (GC/NCIMS) method using headspace solid‐phase microextraction (HS‐SPME) was developed for the determination of trichloroethylene (TCE) in blood, liver, kidney, lung and brain. The method was optimized with respect to several parameters including extraction time, extraction temperature, desorption time and salt addition. The method showed good linearity over the range of 0.025–25 ng/mL in blood and 0.075–75 ng/g in tissues with correlation coefficient (R 2 ) values higher than 0.99. The precision and accuracy for intra‐day and inter‐day measurements were less than 10%. The relative recoveries of all matrices were greater than 52%. Samples showed no significant loss during 8 h in the autosampler and following three freeze/thaw cycles. Validation results demonstrated that selected‐ion monitoring of the 35 Cl and 37 Cl isotopes using NCI resulted in reliable and sensitive quantitation. This validated method was successfully applied to study the toxicokinetics of TCE following oral administration of extremely low doses of this potential human carcinogen to small test animals (rats). Copyright © 2008 John Wiley & Sons, Ltd.