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Structural characterization of constituents with molecular diversity in fractions from Lysidice brevicalyx by liquid chromatography/diode‐array detection/electrospray ionization tandem mass spectrometry and liquid chromatography/nuclear magnetic resonance
Author(s) -
Qu Jing,
Hu Youcai,
Li Jianbei,
Wang Yinghong,
Zhang Jinlan,
Abliz Zeper,
Yu Shishan,
Liu Yunbao
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3419
Subject(s) - chemistry , chromatography , electrospray ionization , characterization (materials science) , mass spectrometry , liquid chromatography–mass spectrometry , electrospray , chromatography detector , direct electron ionization liquid chromatography–mass spectrometry interface , protein mass spectrometry , tandem mass spectrometry , analytical chemistry (journal) , high performance liquid chromatography , ionization , chemical ionization , ion , organic chemistry , nanotechnology , materials science
A combination of electrospray ionization tandem mass spectrometry with high‐performance liquid chromatography (HPLC/ESI‐MS n ), and hyphenation of liquid chromatography to nuclear magnetic resonance spectroscopy (HPLC/NMR), have been extensively utilized for on‐line analysis of natural products, analyzing metabolite and drug impurity. In our last paper, we reported an on‐line analytical method for structural identification of trace alkaloids in the same class. However, the structural types of the constituents in plants were various, such as flavanoids, terpenoids and steroids. It is important to establish an effective analytical method for on‐line structural identification of constituents with molecular diversity in extracts of plants. So, in the present study, the fragmentation patterns of some isolated stilbenes, phloroglucinols and flavanoids from Lysidice rhodostegia were investigated by ESI‐MS n . Their fragmentation rules and UV characteristics are summarized, and the relationship between the spectral characteristics, rules and the structures is described. According to the fragmentation rules, NMR and UV spectral characteristics, 24 constituents of different types in the fractions from L. brevicalyx of the same genus were structurally characterized on the basis of HPLC/HRMS, HPLC‐UV/ESI‐MS n , HPLC/ 1 H NMR and HPLC/ 1 H‐ 1 H COSY rapidly. Of these, six (10, 13, 14, 16, 17 and 23) are new compounds and all of them are reported from L. brevicalyx for the first time. The aim is to develop an effective analytical method for on‐line structural identification of natural products with molecular diversity in plants, and to guide the rapid and direct isolation of novel compounds by chemical screening. Copyright © 2008 John Wiley & Sons, Ltd.