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An electrospray mass spectrometric method for accurate mass determination of highly acid‐sensitive phosphoramidites
Author(s) -
Kupihár Zoltán,
Timár Zoltán,
Darula Zsuzsanna,
Dellinger Douglas J.,
Caruthers Marvin H.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3394
Subject(s) - chemistry , ethylene glycol , electrospray ionization , mass spectrometry , chromatography , electrospray , acetonitrile , ether , peg ratio , extractive electrospray ionization , sample preparation in mass spectrometry , organic chemistry , economics , finance
Abstract An accurate mass determination method utilizing electrospray ionization mass spectrometry is described for analysis of several different types of phosphoramidites that are extremely acid‐sensitive compounds. An earlier method, which applied a LiCl/acetonitrile system, was extended for this special application by using polymeric standards including poly(ethylene glycol) (PEG), poly(ethylene glycol) dimethyl ether (PDE) and poly(propylene glycol) (PPG). Concentrations of standards, samples and LiCl were optimized and potential impurities that affect the analyses were also investigated. Copyright © 2008 John Wiley & Sons, Ltd.