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Electron transfer dissociation in the hexapole collision cell of a hybrid quadrupole‐hexapole Fourier transform ion cyclotron resonance mass spectrometer
Author(s) -
Kaplan Desmond A.,
Hartmer Ralf,
Speir J. Paul,
Stoermer Carsten,
Gumerov Dmitry,
Easterling Michael L.,
Brekenfeld Andreas,
Kim Taeman,
Laukien Frank,
Park Melvin A.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3356
Subject(s) - chemistry , fourier transform ion cyclotron resonance , mass spectrometry , ion cyclotron resonance , electron transfer dissociation , quadrupole ion trap , analytical chemistry (journal) , top down proteomics , ion , ion trap , hybrid mass spectrometer , selected ion monitoring , mass spectrum , dissociation (chemistry) , collision induced dissociation , triple quadrupole mass spectrometer , selected reaction monitoring , tandem mass spectrometry , cyclotron , chromatography , gas chromatography–mass spectrometry , organic chemistry
Abstract Electron transfer dissociation (ETD) of proteins is demonstrated in a hybrid quadrupole‐hexapole Fourier transform ion cyclotron resonance mass spectrometer (Qh‐FTICRMS). Analyte ions are selected in the mass analyzing quadrupole, accumulated in the hexapole linear ion trap, reacted with fluoranthene reagent anions, and then analyzed via an FTICR mass analyzer. The hexapole trap allows for a broad fragment ion mass range and a high ion storage capacity. Using a 3 T FTICRMS, resolutions of 60 000 were achieved with mass accuracies averaging below 1.4 ppm. The high resolution, high mass accuracy ETD spectra provided by FTICR obviates the need for proton transfer reaction (PTR) charge state reduction of ETD product ions when analyzing proteins or large peptides. This is demonstrated with the ETD of ubiquitin and apomyoglobin yielding sequence coverages of 37 and 20%, respectively. We believe this represents the first reported successful combination of ETD and a FTICRMS. Copyright © 2008 John Wiley & Sons, Ltd.