Premium
Detection and quantification of sucrose as dietary biomarker using gas chromatography and liquid chromatography with mass spectrometry
Author(s) -
Kuhnle Gunter G. C.,
Joosen Annemiek M. C. P.,
Wood Tommy R.,
Runswick Shirley A.,
Griffin Julian L.,
Bingham Sheila A.
Publication year - 2008
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3355
Subject(s) - chemistry , chromatography , biomarker , sucrose , mass spectrometry , urine , liquid chromatography–mass spectrometry , urinary system , food science , medicine , biochemistry
Several epidemiological studies suggest a link between the intake of refined sugars and an increased risk for colorectal, breast, pancreatic and endometrial cancer. However, other studies failed to confirm these conclusions and the reason for this may be the ambiguity of dietary assessment methods – mainly self‐reporting – employed. Sucrose is an established biomarker for sugars intake, allowing the objective assessment of dietary sucrose. So far, urinary excretion of sucrose was mainly determined using an enzyme assay. However, this method is time‐consuming and labour‐intensive. In this study, we present a mass spectrometric method for the determination of sucrose in urine using liquid chromatography with mass spectrometry (LC/MS) which can be used for large‐scale epidemiological studies. Copyright © 2008 John Wiley & Sons, Ltd.