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Simultaneous determination of blood glucose and isoleucine levels in rats after chronic alcohol exposure by microwave‐assisted derivatization and isotope dilution gas chromatography/mass spectrometry
Author(s) -
Xue Ruyi,
Zhang Si,
Deng Chunhui,
Dong Ling,
Liu Taotao,
Wang Jiyao,
Wu Hongyi,
Gu Jianxin,
Shen Xizhong
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3350
Subject(s) - chemistry , derivatization , isoleucine , chromatography , isotope dilution , gas chromatography–mass spectrometry , alcohol , mass spectrometry , pyrroloquinoline quinone , amino acid , biochemistry , leucine , enzyme , cofactor
Blood glucose and isoleucine are two biomarkers of chronic alcohol exposure. Simultaneous determination of blood glucose and isoleucine levels helps to illuminate the influence of alcohol on the metabolism of glucose and amino acids. The most accurate method for the detection of serum glucose is isotope dilution gas chromatography/mass spectrometry (ID GC/MS). In this study, a rapid, simple and sensitive technique was developed for the quantitative analysis of glucose and isoleucine in rats after chronic alcohol exposure by microwave‐assisted derivatization (MAD) and ID GC/MS. Serum glucose and isoleucine were rapidly derivatized by N ‐methyl‐ N ‐trimethylsilyltrifluoroacetamide (MSTFA) with microwave irradiation, and the trimethylsilyl derivatives were analyzed by GC/MS. This technique was used to demonstrate that pyrroloquinoline quinone (PQQ), a non‐covalently bound prosthetic group in some quinoproteins involved in the metabolism of some sugar or alcohol, could reverse alcohol exposure induced glucose elevation. On the other hand, it did not affect the metabolism of isoleucine whose level was elevated along with serum glucose. The combination of MAD and ID GC/MS has been shown to be an accurate, rapid, simple and sensitive method for the quantification of glucose and isoleucine in serum samples. Copyright © 2007 John Wiley & Sons, Ltd.