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Simultaneous lipidomic analysis of three families of bioactive lipid mediators leukotrienes, resolvins, protectins and related hydroxy‐fatty acids by liquid chromatography/electrospray ionisation tandem mass spectrometry
Author(s) -
Masoodi Mojgan,
Mir Adnan A.,
Petasis Nicos A.,
Serhan Charles N.,
Nicolaou Anna
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3331
Subject(s) - chemistry , lipidomics , chromatography , electrospray , liquid chromatography–mass spectrometry , mass spectrometry , tandem mass spectrometry , lipid signaling , electrospray ionization , biochemistry , enzyme
Bioactive lipid mediators derived from polyunsaturated fatty acids (PUFA) exhibit a range of tissue‐ and cell‐specific activities in many physiological and pathological processes. Electrospray ionisation tandem mass spectrometry coupled to liquid chromatography (LC/ESI‐MS/MS) is a sensitive, versatile analytical methodology for the qualitative and quantitative analysis of lipid mediators. Here we present an LC/ESI‐MS/MS assay for the simultaneous analysis of twenty mono‐ and poly‐hydroxy‐fatty acid derivatives of linoleic, arachidonic, eicosapentaenoic and docosahexaenoic acids. The assay was linear over the concentration range 1–100 pg/µL, whilst the limits of detection and quantitation were 10–20 and 20–50 pg, respectively. The recovery of the extraction methodology varied from 76–122% depending on the metabolite. This system is useful for profiling a range of biochemically related potent mediators including the newly discovered resolvins and protectins, and their precursor hydroxyeicosapentaenoic and hydroxydocosahexaenoic acids, and, consequently, advance our understanding of the role of PUFA in health and disease. Copyright © 2007 John Wiley & Sons, Ltd.