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Determination of dithiocarbamate fungicide residues by liquid chromatography/mass spectrometry and stable isotope dilution assay
Author(s) -
Crnogorac Goranka,
Schwack Wolfgang
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3312
Subject(s) - chemistry , chromatography , dithiocarbamate , isotope dilution , mass spectrometry , detection limit , extraction (chemistry) , electrospray ionization , ammonium carbonate , analyte , nuclear chemistry , organic chemistry
A rapid and very sensitive high‐performance liquid chromatography/electrospray ionization mass spectrometry (LC/ESI‐MS) method for the simultaneous determination of dithiocarbamate (DTC) fungicide residues in fruits and vegetables was developed. The surface extraction of samples used an alkaline buffer consisting of sodium hydrogen carbonate and DL‐penicillamine. The three DTC subclasses, i.e. dimethyldithiocarbamates (DMDs), ethylenebis(dithiocarbamates) (EBDs), and propylenebis(dithiocarbamates) (PBDs), were separated on a Sequant ZIC‐pHILIC column using an acetonitrile/10 mM ammonia gradient. Because of the instability of DTC residues extracted from plant samples, a stable isotope dilution assay was applied. For each DTC subclass, the limits of detection and quantification were approximately 0.03 mg kg −1 and 0.05 mg kg −1 , respectively. Recoveries from grapes, cucumbers, tomatoes, and rucola, spiked in the range of 0.01–0.9 mg kg −1 , averaged between 90 and 100%. Copyright © 2007 John Wiley & Sons, Ltd.