Premium
Development of a rapid and validated method for investigating the metabolism of scoparone in rat using ultra‐performance liquid chromatography/electrospray ionization quadruple time‐of‐flight mass spectrometry
Author(s) -
Wang Xijun,
Lv Haitao,
Sun Hui,
Liu Lian,
Sun Wenjun,
Cao Hongxin
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3296
Subject(s) - chemistry , chromatography , electrospray ionization , mass spectrometry , electrospray , ionization , direct electron ionization liquid chromatography–mass spectrometry interface , quadrupole time of flight , chemical ionization , organic chemistry , ion
Scoparone (6,7‐dimethoxycoumarin) is known to have a wide range of pharmacological properties. In this study, a rapid and validated ultra‐performance liquid chromatography/electrospray ionization quadruple time‐of‐flight mass spectrometry (UPLC/ESI‐QTof‐MS) method was developed to investigate the metabolism of scoparone in rat for the first time. The new method reduced the sample handling and analytical time by three‐ to six‐fold, and the detection limit by five‐ to 1000‐fold, compared to published methods. Far more metabolites were detected and identified compared to published data, which were preliminarily identified as scopoletin, isoscopoletin, isofraxidin, and fraxidin, respectively, when subjected to tandem mass spectrometry analyses. It is found that the metabolic trajectory of scoparone in rat focused on phase I metabolism which is obviously different from published results, and revealed a wide range of pharmacological properties of scoparone partly attributed to the bioactivities of its metabolites. Copyright © 2007 John Wiley & Sons, Ltd.