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Determination of long‐acting release octreotide, an octapeptide analogue of somatostation, in human plasma by liquid chromatography/tandem mass spectrometry
Author(s) -
Jiang Yao,
Wang Jiang,
Wang Yingwu,
Du Xiaolin,
Zhang Yunhui,
Fawcett J. Paul,
Gu Jingkai
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3293
Subject(s) - chemistry , chromatography , tandem mass spectrometry , electrospray ionization , mass spectrometry , formic acid , liquid chromatography–mass spectrometry , detection limit , selected reaction monitoring , octreotide , high performance liquid chromatography , dichloromethane , somatostatin , biochemistry , neuroscience , solvent , biology
A sensitive and selective method for the determination of long‐acting released octreotide in human plasma has been developed based on liquid chromatography/tandem mass spectrometry (LC/MS/MS). Octreotide and the internal standard, triptorelin, were precipitated from the matrix, washed with dichloromethane and subsequently separated by reversed‐phase high‐performance liquid chromatography (HPLC) employing a 1% formic acid/methanol gradient system. Detection was by electrospray ionization mass spectrometry in the positive ion mode using multiple‐reaction monitoring. The assay was linear in the concentration range 0.0500–50.0 ng/mL with intra‐ and inter‐day precision (as relative standard deviation) of <2.95% and <8.37%, respectively. The limit of detection was 0.0200 ng/mL. The method was applied to a pharmacokinetic study of long‐acting released octreotide in healthy volunteers given an intramuscular injection containing 20 mg octreotide. Copyright © 2007 John Wiley & Sons, Ltd.