Premium
Expanding the linear dynamic range in quantitative high performance liquid chromatography/tandem mass spectrometry by the use of multiple product ions
Author(s) -
Curtis Michael A.,
Matassa Luca C.,
Demers Roger,
Fegan Katrina
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.327
Subject(s) - calibration curve , chemistry , bioanalysis , chromatography , calibration , analytical chemistry (journal) , tandem mass spectrometry , linearity , mass spectrometry , tandem , high performance liquid chromatography , liquid chromatography–mass spectrometry , range (aeronautics) , dynamic range , sensitivity (control systems) , ion , selected reaction monitoring , detection limit , materials science , optics , statistics , physics , mathematics , organic chemistry , quantum mechanics , electronic engineering , engineering , composite material
A strategy for expanding the linear working range in bioanalysis using quantitative high performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) is presented. The strategy involves monitoring multiple product ions. Herein we demonstrate the strategy on a rat plasma assay for a proprietary experimental drug where the linear range is expanded from 2 to 4 orders of magnitude. A primary sensitive ion was monitored to obtain a high sensitivity range calibration curve (0.400 to 100 ng/mL) while a less sensitive secondary ion was monitored to obtain a low sensitivity range calibration curve (90.0 to 4000 ng/mL). Each calibration curve gave acceptable linearity (r >0.990). Quality control samples at low, mid and high levels within each calibration curve demonstrated acceptable precision and accuracy (within 20% for all levels). The technique was successfully applied to rat pre‐clinical sample analysis. Copyright © 2001 John Wiley & Sons, Ltd.