Premium
High‐field asymmetric waveform ion mobility spectrometry coupled with liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐FAIMS‐MS/MS) multi‐component bioanalytical method development, performance evaluation and demonstration of the constancy of the compensation voltage with change of mobile phase composition or flow rate
Author(s) -
Wu Steven T.,
Xia YuanQing,
Jemal Mohammed
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3264
Subject(s) - chemistry , chromatography , mass spectrometry , ion mobility spectrometry , bioanalysis , electrospray ionization , electrospray , analytical chemistry (journal) , tandem mass spectrometry , analyte , liquid chromatography–mass spectrometry
The feasibility of developing a multi‐component bioanalytical method using high‐field asymmetric waveform ion mobility spectrometry coupled with liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐FAIMS‐MS/MS) is demonstrated using nefazodone and its two metabolites as model compounds. The performance of the bioanalytical method for the three analytes, with three different compensation voltage (CV) values, is assessed using standard curves and quality control samples, which exhibited good accuracy, precision and ruggedness. The number of analytes with different CV values that can be quantitated simultaneously depends on the acquisition cycle time, which is a function of the FAIMS residence time (fixed), chromatographic peak width and selected reaction monitoring (SRM) dwell time. It is established that CV, the FAIMS selectivity parameter, is reproducible for at least 16 h, thus ensuring the constancy of the CV during a large‐batch sample analysis. It is also established that change in mobile phase composition or of flow rate does not cause a shift in CV. Thus, CV values determined from a CV scan via infusion of a sample can be used for an LC/ESI‐FAIMS‐M/MS method based on isocratic or gradient elution. Copyright © 2007 John Wiley & Sons, Ltd.