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Aldehyde analysis by high performance liquid chromatography/tandem mass spectrometry
Author(s) -
O'BrienCoker Ivan C.,
Perkins George,
Mallet Anthony I.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.324
Subject(s) - chemistry , chromatography , tandem mass spectrometry , mass spectrometry , aldehyde , tandem , liquid chromatography–mass spectrometry , quantitative analysis (chemistry) , selected reaction monitoring , high performance liquid chromatography , organic chemistry , materials science , composite material , catalysis
This paper describes the development of a high performance liquid chromatography/tandem mass spectrometric (MS/MS) procedure for the specific qualitative and quantitative analysis of lipid aldehydes in biological matrices. A derivatisation method, which results in molecules that exhibit a common product ion on MS/MS, permits informative precursor ion scans, at high sensitivity. This has been applied to the examination of plasma in order to examine the production of aldehydes consequent on in vitro lipid oxidation. Quantitative analysis of target molecules using multiple reaction monitoring has been developed to permit quantitation in the same matrices. Copyright© 2001 John Wiley & Sons, Ltd.