Premium
Application of the statistical test of equivalent pathways (STEP) method to the triple quadrupole mass spectrometer
Author(s) -
Dalpathado Dilusha S.,
Chang Qing,
Burkett Colleen M.,
Bandu Mary L.,
Desaire Heather
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3229
Subject(s) - chemistry , triple quadrupole mass spectrometer , mass spectrometry , quadrupole mass analyzer , quadrupole , hybrid mass spectrometer , spectrometer , analytical chemistry (journal) , chromatography , selected reaction monitoring , atomic physics , tandem mass spectrometry , physics , quantum mechanics
Recently, we demonstrated a new method, STEP (Statistical Test of Equivalent Pathways) analysis, which differentiates first‐generation product ions (primary product ions) from second‐generation product ions (secondary product ions) obtained in tandem mass spectrometric (MS/MS) experiments on a quadrupole ion trap mass spectrometer. The study presented here defines how to adapt the STEP method to a more routinely used mass analyzer, the triple quadrupole. New ion activation conditions were developed to adapt the STEP method to the triple quadrupole mass spectrometer using peptides and carbohydrates. The application of this method to the triple quadrupole is useful because it provides an efficient approach to differentiate primary and secondary ions on this instrument. Out of the total number of ions that were subjected to the STEP analysis, this method correctly identified 96% of ions as primary or secondary, indicating that this analysis is effective for carbohydrates and peptides undergoing collision‐induced dissociation (CID) on a triple quadrupole mass spectrometer. Copyright © 2007 John Wiley & Sons, Ltd.