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Determination of gatifloxacin in human plasma by liquid chromatography/electrospray tandem mass spectrometry
Author(s) -
Vishwanathan Karthick,
Bartlett Michael G.,
Stewart James T.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.322
Subject(s) - chemistry , gatifloxacin , chromatography , selected reaction monitoring , mass spectrometry , electrospray ionization , liquid chromatography–mass spectrometry , electrospray , tandem mass spectrometry , detection limit , analyte , ciprofloxacin , biochemistry , antibiotics
Gatifloxacin is an advanced‐generation, 8‐methoxyfluoroquinolone that is active against a broad spectrum of pathogens, including antiobiotic resistant Streptococcus pneumoniae . Development of a rapid, sensitive and selective method for the determination of gatifloxacin in human plasma is essential for understanding the pharmacokinetics of the drug when administered orally or intravenously. Solid phase extraction (SPE) using Oasis® HLB was used to extract gatifloxacin and the internal standard ciprofloxacin from plasma. A method based on liquid chromatography/electrospray tandem mass spectrometry (LC/ESI‐MS/MS) was developed and validated to quantitate gatifloxacin in human plasma. The precursor and major product ions of the analyte were monitored on a triple quadrupole mass spectrometer with positive ion electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. Mechanisms for the formation of collision‐induced dissociation products of gatifloxacin are proposed. Linear calibration curves were generated from 10–1000 ng/mL with coefficients of determination greater than 0.99. The interday and intraday precision (%RSD) was less than 6.0% and accuracy (%error) was less than 5.4% for gatifloxacin. The limit of detection (LOD) for the method was 500 pg/mL based on a signal‐to‐noise ratio of 3. Copyright © 2001 John Wiley & Sons, Ltd.