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Analysis of low molecular weight acids by negative mode matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Shroff Rohit,
Muck Alexander,
Svatoš Aleš
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3216
Subject(s) - chemistry , mass spectrometry , chromatography , salicylic acid , jasmonic acid , desorption , ionization , matrix assisted laser desorption/ionization , matrix (chemical analysis) , analytical chemistry (journal) , time of flight mass spectrometry , chemical ionization , organic chemistry , ion , biochemistry , adsorption
Free 9‐aminoacridine base is demonstrated to be a suitable matrix for negative mode matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometric (MALDI‐TOFMS) analysis of a wide range of low molecular weight organic acids including aliphatic (from acetic to palmitic acid), aromatic acids, phytohormones (e.g. jasmonic and salicylic acids), and amino acids. Low limits of quantitation in the femtomolar range (jasmonic – 250 fmol; caffeic – 160 fmol and salicylic – 12.5 fmol) and linear detector response over two concentration orders in the pico‐ and femtomolar range are extremely encouraging for the direct study of such acids in complex biological matrices. Copyright © 2007 John Wiley & Sons, Ltd.