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Simultaneous determination of aflatoxins, ochratoxin A and Fusarium toxins in maize by liquid chromatography/tandem mass spectrometry after multitoxin immunoaffinity cleanup
Author(s) -
Lattanzio Veronica Maria Teresa,
Solfrizzo Michele,
Powers Steve,
Visconti Angelo
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3210
Subject(s) - chemistry , chromatography , ochratoxin a , aflatoxin , mycotoxin , zearalenone , mass spectrometry , tandem mass spectrometry , liquid chromatography–mass spectrometry , ammonium acetate , extraction (chemistry) , ochratoxin , electrospray ionization , detection limit , solid phase extraction , high performance liquid chromatography , food science
A liquid chromatography/tandem mass spectrometry method was developed for the simultaneous determination of aflatoxins (B 1 , B 2 , G 1 , G 2 ), ochratoxin A, fumonisins (B 1 , B 2 ), deoxynivalenol, zearalenone, T‐2 and HT‐2 toxins in maize. A double extraction approach, using a phosphate‐buffered solution followed by methanol, was applied to achieve effective co‐extraction of the 11 mycotoxins under investigation having quite different polarities and chemical structures. A new multitoxin immunoaffinity column containing antibodies for all these mycotoxins was used to clean up the extract. Detection and quantification of the 11 mycotoxins were performed by reversed‐phase liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry (LC/ESI‐MS/MS) using, as chromatographic mobile phase, a linear gradient of methanol/water containing 0.5% acetic acid and 1 mM ammonium acetate. Method performances were quite satisfactory for all tested mycotoxins at contamination levels close to or below the relevant EU maximum permitted or recommended levels. Limits of detection in maize ranged from 0.3 to 4.2 µg/kg. Recoveries higher than 79% were obtained for all tested mycotoxins with relative standard deviations less than 13%. Copyright © 2007 John Wiley & Sons, Ltd.