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Liquid chromatography/atmospheric pressure chemical ionization mass spectrometry of terpene lactones in plasma of volunteers dosed with Ginkgo biloba L. extracts
Author(s) -
Mauri Pierluigi,
Simonetti Paolo,
Gardana Claudio,
Minoggio Markus,
Morazzoni Paolo,
Bombardelli Ezio,
Pietta Piergiorgio
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.316
Subject(s) - ginkgolides , chemistry , atmospheric pressure chemical ionization , chromatography , ginkgo biloba , ginkgoales , mass spectrometry , high performance liquid chromatography , chemical ionization , liquid chromatography–mass spectrometry , terpene , pharmacognosy , ionization , organic chemistry , biological activity , biochemistry , pharmacology , medicine , ion , in vitro
Liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (LC/APCI‐ITMS) was applied to evaluate the levels of ginkgolides A and B and bilobalide in plasma of volunteers after administration of Ginkgo biloba extracts in free (Ginkgoselect®) or phospholipid complex (Ginkgoselect® Phytosome®) forms, providing 9.6 mg of total terpene lactones. The maximum plasma concentrations, C max , of total ginkgolides A, B and bilobalide were 85.0 and 181.8 µg/mL for Ginkgoselect and Ginkgoselect Phytosome, respectively. The C max values were reached at 120 min for the free form and at 180–240 min for the phospholipid complex form. In both cases, the mean elimination half‐life of each terpene lactone was in the range 120–180 min. Due to its sensitivity (about 1 ng/mL) and specificity, LC/APCI‐ITMS proved to be a very powerful tool for pharmacokinetic studies of these phytochemicals. Copyright © 2001 John Wiley & Sons, Ltd.