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Membrane proteome in Escherichia coli probed by MS 3 mass spectrometry: a preliminary report
Author(s) -
Cirulli Claudia,
Marino Gennaro,
Amoresano Angela
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3104
Subject(s) - chemistry , proteome , escherichia coli , bacterial outer membrane , membrane protein , transmembrane protein , bacteria , biochemistry , mass spectrometry , membrane , tandem mass spectrometry , tandem mass tag , proteomics , cell membrane , bacterial cell structure , computational biology , chromatography , quantitative proteomics , biology , genetics , receptor , gene
Characterization of the membrane proteome is particularly intriguing since a better knowledge in this field might lead to new insights into the function of different membrane systems. Despite the biological relevance of surface proteins however, their characterization still remains a challenging task. Outer membrane proteins (OMPs) of Gram‐negative bacteria are key molecules that interface the cell with the environment. Hence, surface proteins of Gram‐negative bacteria contain proteins that might be good targets for drugs, antimicrobials or detection systems and they may become components of effective vaccines. In this respect, Escherichia coli has been chosen as a model organism for several structural and functional studies aimed at understanding the biophysical and biochemical organization of proteins in Gram‐negative cell walls. Here we present first results for the identification of bacterial surface exposed proteins in E. coli K12 based on the use of dansyl chloride labelling coupled with bidimensional tandem mass spectrometry exploiting the advantage of precursor ion/MS 3 scan modes. This procedure resulted in a promising, simple, and rapid strategy for the identification of membrane proteins in E. coli as model organism, thus avoiding time‐consuming procedures based on two‐dimensional liquid chromatography and electrophoresis. The proteins identified could be grouped into five major families: outer membrane (29 proteins), lipoproteins (6 proteins), transmembrane (43 proteins) families. Copyright © 2007 John Wiley & Sons, Ltd.