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An inductively coupled plasma mass spectrometry method for the quantification of yttrium‐antibody based drugs using stable isotope tracing
Author(s) -
Ciavardelli Domenico,
D'Anniballe Gaetano,
Nano Giuseppe,
Martin Franck,
Federici Giorgio,
Sacchetta Paolo,
Di Ilio Carmine,
Urbani Andrea
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3094
Subject(s) - chemistry , inductively coupled plasma mass spectrometry , tracing , yttrium , chromatography , mass spectrometry , stable isotope ratio , isotope , analytical chemistry (journal) , organic chemistry , physics , computer science , operating system , oxide , quantum mechanics
Abstract Targeted radioimmunotherapy has been recently clinically validated and approved for the treatment of cancer by the US Food and Drug Administration. This therapeutic approach employs monoclonal antibodies directed to cancer‐related, cell‐surface antigens coupled to β‐emitting nuclides. 90 Y is one of the most useful radioisotopes in the development of antibody based radioimmunotherapy and evaluation of the pharmacokinetic profile for 90 Y‐radiopharmaceuticals is usually performed by radiochemical methods. In this work we have developed an alternative radioactive‐free approach to evaluate pharmacokinetic profiles based on the inductively coupled plasma mass spectrometric (ICP‐MS) quantification of 89 Y. A highly sensitive and rapid method for the determination of yttrium in urine is described and applied to evaluate the urinary clearance of antibody‐based drugs labeled with the stable isotope of yttrium, 89 Y. This approach overcomes some important limitations for pre‐clinical radioanalytical methods such as radiation hazards and radioactive waste disposal. Method development was performed by determining detection and quantification limits, and precision as repeatability and trueness. These performance parameters fulfilled the acceptance criteria for bioanalytical methods. Copyright © 2007 John Wiley & Sons, Ltd.