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Simultaneous analysis of multiple bioactive constituents in Rheum tanguticum Maxim. ex Balf. by high‐performance liquid chromatography coupled to tandem mass spectrometry
Author(s) -
Jin Wei,
Wang YingFeng,
Ge RiLi,
Shi HaiMing,
Jia CunQin,
Tu PengFei
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3086
Subject(s) - chemistry , chromatography , mass spectrometry , mass spectrum , electrospray ionization , high performance liquid chromatography , tandem mass spectrometry , electrospray , chemical constituents , analytical chemistry (journal)
A simple, rapid method was developed using on‐line high‐performance liquid chromatography (HPLC)–diode array detection (DAD)/electrospray ionization mass spectrometry (ESI‐MS) to simultaneously analyze multiple bioactive constituents in the extract of Rheum tanguticum Maxim. ex Balf. (Dahuang), a traditional Chinese medicine (TCM). Many bioactive constituents gave prominent [MH] − ions in the negative ion ESI mass spectra. Among them, 41 different constituents including 16 anthraquinone derivatives, 7 phenylbutanone glucopyranosides, 4 stilbenes and 14 tannins were unambiguously identified or tentatively characterized based on their retention times, UV spectra and mass spectra in comparison with the data from standards or references. Meanwhile, some principles of fragmentation behavior for different types of constituents were proposed, which could contribute to the elucidation of these constituents in Rheum tanguticum . Copyright © 2007 John Wiley & Sons, Ltd.