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Analysis of a chlorosulfolipid from Ochromonas danica by matrix‐assisted laser desorption/ionization quadrupole ion trap time‐of‐flight mass spectrometry
Author(s) -
Darsow Kai H.,
Lange Harald A.,
Resch Martin,
Walter Christian,
Buchholz Rainer
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3076
Subject(s) - chemistry , mass spectrometry , analytical chemistry (journal) , quadrupole ion trap , ionization , fragmentation (computing) , field desorption , desorption , ion , time of flight mass spectrometry , matrix assisted laser desorption/ionization , soft laser desorption , ion trap , ion source , polyatomic ion , chromatography , organic chemistry , adsorption , computer science , operating system
Matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight (ToF) mass spectrometry (MS) is an established tool for analyzing high mass molecules, such as proteins, whereas it attracts far less interest in the field of lipid analysis. In the study reported here a new chlorosulfolipid (CSL), 3,8,12,15‐tetrachloroeicosane‐1,17,18‐triyl tris(hydrogen sulfate), was identified from the alga Ochromonas danica and de novo characterized by matrix‐assisted laser desorption/ionization quadrupole ion trap time‐of‐flight (MALDI‐QIT‐ToF) MS in negative ion mode. This method provides an effective alternative for the analysis of compounds directly derived from organic cell extracts. For MALDI analyses several frequently used solid MALDI matrices as well as some ionic liquid matrices (ILMs) were tested to enhance the analyte response to UV‐laser and its ionization. The molecular weight of the observed compound could be determined as Li‐, Na‐ and K‐adducts [M+Me2H] − . The characteristic isotopic patterns of the measured ions and the well‐allocated molecular fragments by MS 1 , MS 2 and MS 3 indicate the fourfold chlorination and threefold sulfation of the investigated compound. The MS fragmentation alongside of the chlorine‐bearing C‐atoms is accompanied by the generation of a double bond at the opposite fragment in MS 1 . This obtained fragmentation pattern provides an insight into the allocation of the chlorine‐bearing C‐atoms along the carbon chain. Copyright © 2007 John Wiley & Sons, Ltd.

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