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Matrix‐assisted laser desorption/ionization mass spectrometry of polysaccharides with 2′,4′,6′‐trihydroxyacetophenone as matrix
Author(s) -
Hsu NienYeen,
Yang WenBin,
Wong ChiHuey,
Lee YuanChuan.,
Lee Reiko T.,
Wang YiSheng,
Chen ChungHsuan
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3072
Subject(s) - chemistry , polysaccharide , mass spectrometry , desorption , matrix assisted laser desorption/ionization , matrix (chemical analysis) , polysialic acid , molecular mass , ionization , chromatography , biochemistry , organic chemistry , ion , cell adhesion , enzyme , adsorption , cell , neural cell adhesion molecule
Abstract So far, there have been only a few matrices reported for detection of polysaccharides with molecular weight higher than 3000 Daltons by matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS). In this work, we found that 2′,4′,6′‐trihydroxyacetophenone (THAP) is a good matrix for MALDI time‐of‐flight MS analysis of polysaccharides with broad mass range. Large polysaccharides, dextrans, glycoproteins and polysialic acids have been successfully detected by MALDI‐MS with THAP as matrix. Copyright © 2007 John Wiley & Sons, Ltd.