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Enumeration of carbohydrate hydroxyl groups by silylation and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Adeuya Anthony,
Price Neil P. J.
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3067
Subject(s) - chemistry , analyte , mass spectrometry , chromatography , desorption , silylation , trimethylsilyl , analytical chemistry (journal) , ionization , mass spectrum , ion , organic chemistry , catalysis , adsorption
A method for enumerating hydroxyl groups in analytes is described and applied to various carbohydrates. The analytes were derivatized in solution by using trimethylsilylimidazole (TMSI) and the products were analyzed without chromatography by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS). The mass spectra revealed consecutive product ion peaks that are separated by 72 m/z units. This 72 m/z unit separation is a result of displacement of a hydroxyl hydrogen atom by a trimethylsilyl group. The number of the observed ions was used to confirm the number of hydroxyl groups in each analyte. This method was utilized in the analysis of single and multi‐component analytes. Published in 2007 by John Wiley & Sons, Ltd.