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Determining enediol compounds in tea using surface‐assisted laser desorption/ionization mass spectrometry with titanium dioxide nanoparticle matrices
Author(s) -
Lee KunHong,
Chiang ChengKang,
Lin ZongHong,
Chang HuanTsung
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3058
Subject(s) - chemistry , titanium dioxide , desorption , mass spectrometry , repeatability , nanoparticle , chromatography , ascorbic acid , sample preparation , detection limit , matrix assisted laser desorption/ionization , analytical chemistry (journal) , adsorption , organic chemistry , chemical engineering , food science , engineering
We describe the use of titanium dioxide nanoparticles (TiO 2 NPs) as selective probes and matrices for the determination of catechins using surface‐assisted laser desorption/ionization mass spectrometry (SALDI‐MS). The interactions between the enediol compounds and TiO 2 NPs were evident by the change in color of the TiO 2 NP solution from milky white to orange. Through these interactions, the TiO 2 NPs could be used to concentrate enediol compounds, including catechins and ascorbic acid. The limits of detection (LODs) for three catechins – catechin, (−)‐epigallocatechin, and (−)‐epigallocatechin gallate – at a signal‐to‐noise ratio of 3 were 0.45, 1.85 and 0.65 µM, respectively. The TiO 2 NP matrices provide a number of advantages over conventional organic matrices (e.g. 2′,4′,6′‐trihydroxyacetophenone), including ease of sample preparation, less background noise in the low‐mass region, and high repeatability. The applicability of this method was confirmed through the high reproducibility of the determination of the two catechins in tea samples that had not been subjected to any sample preparation procedures (shot‐to‐shot variation: <10%). Copyright © 2007 John Wiley & Sons, Ltd.