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Direct analysis of isopropylthioxanthone (ITX) in milk by high‐performance liquid chromatography/tandem mass spectrometry
Author(s) -
Bagnati Renzo,
Bianchi Giancarlo,
Marangon Elena,
Zuccato Ettore,
Fanelli Roberto,
Davoli Enrico
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3055
Subject(s) - chemistry , chromatography , tandem mass spectrometry , mass spectrometry , contamination , high performance liquid chromatography , liquid chromatography–mass spectrometry , sample preparation , acetonitrile , biology , ecology
A fast screening method is presented for detecting isopropylthioxanthone (ITX) contamination in milk. The method is based on direct high‐performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) analysis of milk samples. Sample preparation is limited to the addition of a deuterated ITX solution in acetonitrile that serves both as internal standard and to precipitate proteins. The method is highly accurate and sensitive. Isomeric specific analyses of 2‐ITX and 4‐ITX are possible at 6 µg/L levels with about 5% precision and accuracy. This approach has been used to check contamination in samples like milk, soy milk, baby milk, in their packaging material. Out of 37 milk samples analyzed, 16 were positive with concentrations ranging from 173–439 µg/L for 2‐ITX and from <6 (lower than limit of quantification) to 25 µg/L for 4‐ITX. Copyright © 2007 John Wiley & Sons, Ltd.