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Flavonoid profile in soybeans by high‐performance liquid chromatography/tandem mass spectrometry
Author(s) -
Cavaliere Chiara,
Cucci Francesca,
Foglia Patrizia,
Guarino Chiara,
Samperi Roberto,
Laganà Aldo
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3049
Subject(s) - chemistry , chromatography , tandem mass spectrometry , flavonoid , mass spectrometry , tandem , liquid chromatography–mass spectrometry , organic chemistry , antioxidant , materials science , composite material
Flavonoid profiling of a soybean sample has been performed by liquid chromatography/positive electrospray tandem mass spectrometry (LC/ESI + ‐MS/MS) using a quadrupole‐linear ion trap (QLIT) instrument with an information‐dependent data acquisition (IDA) protocol that looped, in a single run, an enhanced MS scan and an enhanced product ion scan. As compromise between time and obtainable information, spectra acquisition was split into two distinct runs: 220–450 Th and 400–800 Th, respectively. The isoflavones daidzein and genistein were identified as aglycones, monoglycosides, diglycosides, triglycosides, acetylglycosides, malonylglycosides, malonyl diglycosides, and dimalonyl diglycosides, whereas glycitein triglycosides, acetylglycosides, and dimalonyl diglycosides were not detected. Also kaempferol di‐ and triglycosides, malonylglycosides and malonyl diglycosides, previously reported in soy leaves and pods, and four naringenin malonylglycosides were identified. Copyright © 2007 John Wiley & Sons, Ltd.