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Factors influencing enantiomeric fractions of hexabromocyclododecane measured using liquid chromatography/tandem mass spectrometry
Author(s) -
Marvin Chris H.,
MacInnis Gordia,
Alaee Mehran,
Arsenault Gilles,
Tomy Gregg T.
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3040
Subject(s) - hexabromocyclododecane , chemistry , enantiomer , chromatography , mass spectrometry , diastereomer , tandem mass spectrometry , organic chemistry , fire retardant
Hexabromocyclododecane (HBCD), the most heavily produced of the cycloaliphatic brominated flame retardants (BFRs), is a mixture of three predominant diastereomers ( α ‐, β ‐, and γ ‐HBCD), each with a corresponding pair of enantiomers. We have investigated the liquid chromatography/mass spectrometry (LC/MS) behaviour of the HBCD enantiomers, and demonstrated that enantiomeric fractions (EFs) calculated using data uncorrected for instrument and/or matrix effects can result in potentially inaccurate EF values. However, use of labelled surrogates effectively corrects for these effects. Experiments with racemic HBCD standards indicate that chromatographic factors, including mobile phase composition and column bleed from chiral stationary phases, may be contributors to variations in the mass spectrometric response of the HBCD enantiomers. Copyright © 2007 John Wiley & Sons, Ltd.