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Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa
Author(s) -
Rodrigues Clenilson M.,
Rinaldo Daniel,
dos Santos Lourdes C.,
Montoro Paola,
Piacente Sonia,
Pizza Cosimo,
HirumaLima Clélia A.,
Brito Alba R. M. Souza,
Vilegas Wagner
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3036
Subject(s) - chemistry , chromatography , electrospray ionization , electrospray , tandem mass spectrometry , mass spectrometry , proanthocyanidin , polyphenol , organic chemistry , antioxidant
Direct flow injection electrospray ionization ion trap tandem mass spectrometry (ESI‐IT‐MS/MS) was used to investigate the polyphenolic compounds present in an infusion from the barks of Hancornia speciosa Gom. (Apocynaceae), a native Brazilian plant popularly known as ‘mangabeira’, used as a source of nutrition and against gastric disorders. After a simple sample filtration pretreatment the characteristic fingerprint of the infusion was performed in negative ion ESI mode in a few minutes. At low capillary‐voltage activation, the deprotonated molecules ([MH] − ) were observed and using collision‐induced dissociation the product ion spectra showed the presence of a homologous series of B‐type proanthocyanidins, as well as another series containing their respective C‐glycosylated derivatives, with a degree of polymerization from 1 up to 6 units of interlinked catechins. Therefore, direct flow injection allowed us to identify the key compounds without preparative isolation of the components. Copyright © 2007 John Wiley & Sons, Ltd.