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Investigation of unknown related substances in commercial neomycin samples with liquid chromatography/ion trap tandem mass spectrometry
Author(s) -
Li Bo,
Van Schepdael Ann,
Hoogmartens Jos,
Adams Erwin
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3030
Subject(s) - chemistry , chromatography , mass spectrometry , chromatography detector , impurity , ion trap , tandem mass spectrometry , liquid chromatography–mass spectrometry , analytical chemistry (journal) , high performance liquid chromatography , organic chemistry
The characterization of unknown impurities present in neomycin sulfate by liquid chromatography (LC) coupled with ion trap mass spectrometry (ITMS) is described. The volatile LC method was developed using an evaporative light scattering detector due to its lower investment and operating costs, easier operation and less maintenance than mass spectrometry. The method shows separation of neomycin B from seven potential related substances reported in the European Pharmacopoeia and several other unknown impurities. The unknown impurities were further investigated by coupling the developed LC method with ITMS. Their structures were deduced based on the fragmentation patterns obtained from reference substances. Four unknowns were identified as isomers of paromamine, LP‐A, neamine and LP‐B. Copyright © 2007 John Wiley & Sons, Ltd.