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High‐performance liquid chromatography‐diode array detection/electrospray ionization mass spectrometry for the simultaneous analysis of cis‐, trans‐ and dihydro‐2‐glucosyloxycinnamic acid derivatives from Dendrobium medicinal plants
Author(s) -
Yang Li,
Nakamura Norio,
Hattori Masao,
Wang Zhengtao,
Bligh S. W. Annie,
Xu Luoshan
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3025
Subject(s) - chemistry , chromatography , electrospray ionization , mass spectrometry , high performance liquid chromatography , electrospray , dendrobium , mass spectrum , fragmentation (computing) , computer science , operating system , botany , biology
Sensitive, selective and reliable high‐performance liquid chromatography (HPLC)‐diode array detection (DAD)/electrospray ionization multi‐stage mass spectrometry (ESI‐MS n ) methods have been developed for the characterization of nine 2‐glucosyloxycinnamic acid derivatives and quantitative analysis of three of the major 2‐glucosyloxycinnamic acids, cis ‐melilotoside, trans ‐melilotoside and dihydromelilotoside, present in Dendrobium medicinal plants. The identities of the latter three major 2‐glucosyloxycinnamic acids were confirmed by comparing their retention times, UV and mass spectra with those of the reference standards. The characteristic ESI‐MS n fragmentation patterns of the remaining six 2‐glucosyloxycinnamic acid derivatives, which are similar to the three major compounds, have allowed the putative elucidation of their structures. The concentrations of the cis ‐, trans ‐ and dihydromelilotosides were simultaneously determined by HPLC/ESI‐MS 2 using the multiple reaction monitoring (MRM) mode in extracts of Dendrobium species. The method was validated with respect to the overall intra‐ and inter‐day variation (RSD less than 8%) and the limits of quantification for the cis ‐, trans ‐ and dihydromelilotosides were 0.09, 0.09 and 0.01 µg/mL, respectively. Copyright © 2007 John Wiley & Sons, Ltd.