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Determination of 30 elements in colorectal biopsies by sector field inductively coupled plasma mass spectrometry: method development and preliminary baseline levels
Author(s) -
Bocca Beatrice,
Lamazza Antonella,
Pino Anna,
De Masi Ercole,
Iacomino Maura,
Mattei Daniela,
Rahimi Siavash,
Fiori Enrico,
Schillaci Alberto,
Alimonti Alessandro,
Forte Giovanni
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3016
Subject(s) - chemistry , inductively coupled plasma mass spectrometry , analytical chemistry (journal) , microwave digestion , inductively coupled plasma , isotope , metal , mass spectrometry , detection limit , chromatography , plasma , physics , organic chemistry , quantum mechanics
An analytical procedure applicable to restricted sample sizes was developed and applied to the analysis of 30 chemical elements in colorectal biopsies of healthy patients. Acidic microwave digestion processed ≤10 mg of tissue at 80°C in 15‐mL polystyrene liners. The digests were diluted to a volume of 2 mL with high‐purity water and directly analyzed by sector field inductively coupled plasma mass spectrometry without further specimen handling. A careful selection of isotopes and instrumental resolution permitted the quantification in a single analytical sequence both of the elements present at parts per trillion and of those at parts per million. The accuracy calculated on BCR 184 ranged from 93.3–110%, the recoveries of the biopsy material was in the range 95.2–105%, the precision was <10%, and the blank levels were much below those expected in biopsy samples. The metal concentrations (on a dry‐weight basis) in colorectal normal tissue showed a large range of variation: Ag, Au, Be, Bi, Co, Li, Sb, Tl, V, W and Zr were below 50 ng g −1 ; As, Ba, Cd, Cr, Cs, Hg, Mo, Ni, Pb, Se and Sn were distributed from 100 to 500 ng g −1 ; Al, Cu, Fe, Mn, Sr and Zn were from a few µg g −1 to 100 µg g −1 ; and Ca and Mg were at a level of 1000 µg g −1 . These data represent the first attempt to achieve an elemental profile in the colorectal mucosa of healthy patients as baseline level measurements for studies focused on the imbalance of chemical elements in diseased mucosa. Copyright © 2007 John Wiley & Sons, Ltd.