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Lipid composition of membranes of Escherichia coli by liquid chromatography/tandem mass spectrometry using negative electrospray ionization
Author(s) -
Oursel Delphine,
LoutelierBourhis Corinne,
Orange Nicole,
Chevalier Sylvie,
Norris Victor,
Lange Catherine M.
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.3013
Subject(s) - chemistry , chromatography , electrospray ionization , mass spectrometry , phospholipid , tandem mass spectrometry , protein mass spectrometry , liquid chromatography–mass spectrometry , sample preparation in mass spectrometry , extractive electrospray ionization , mass spectrum , electrospray , escherichia coli , membrane , biochemistry , gene
A liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) method using reversed‐phase chromatography was developed for the analysis of phospholipids from bacterial extracts of a wild‐type strain of Escherichia coli . Product ion mass spectra from [MH] − precursor ions allowed an identification of individual phospholipid species that includes both fatty acid composition and fatty acyl location on the glycerol backbone using diagnostic product ions. Thus, complete assignment, including sn ‐1/ sn ‐2 fatty acyl position, was achieved for this strain of E . coli . In addition, the phospholipids were quantified relative to one another using an internal standard method. Copyright © 2007 John Wiley & Sons, Ltd.