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Performance evaluation of elemental analysis/isotope ratio mass spectrometry methods for the determination of the D/H ratio in tetramethylurea and other compounds – results of a laboratory inter‐comparison
Author(s) -
Bréas Olivier,
Thomas Freddy,
Zeleny Reinhard,
Calderone Giovanni,
Jamin Eric,
Guillou Claude
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2988
Subject(s) - tetramethylurea , chemistry , isotope ratio mass spectrometry , mass spectrometry , reproducibility , certified reference materials , analytical chemistry (journal) , chromatography , fractionation , repeatability , detection limit , organic chemistry , solvent
Tetramethylurea (TMU) with a certified D/H ratio is the internal standard for Site‐specific Natural Isotope Fractionation measured by Nuclear Magnetic Resonance (SNIF‐NMR®) analysis of wine ethanol for detection of possible adulterations (Commission Regulation 2676/90). A new batch of a TMU certified reference material (CRM) is currently being prepared. Whereas SNIF‐NMR® has been employed up to now, Elemental Analysis/Isotope Ratio Mass Spectrometry ( 2 H‐EA‐IRMS) was envisaged as the method of choice for value assignment of the new CRM, as more precise (better repeatable) data might be obtained, resulting in lower uncertainty of the certified value. In order to evaluate the accuracy and intra‐ and inter‐laboratory reproducibility of 2 H‐EA‐IRMS methods, a laboratory inter‐comparison was carried out by analysing TMU and other organic compounds, as well as some waters. The results revealed that experienced laboratories are capable of generating robust and well comparable data, which highlights the emerging potential of IRMS in food authenticity testing. However, a systematic bias between IRMS and SNIF‐NMR® reference data was observed for TMU; this lack of data consistency rules out the 2 H‐IRMS technique for the characterisation measurement of the new TMU CRM. Copyright © 2007 John Wiley & Sons, Ltd.