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Laser‐induced photofragmentation of neutral and acidic glycans inside an ion‐trap mass spectrometer
Author(s) -
Devakumar Arugadoss,
Mechref Yehia,
Kang Pilsoo,
Novotny Milos V.,
Reilly James P.
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2981
Subject(s) - chemistry , glycan , fragmentation (computing) , photodissociation , quadrupole ion trap , mass spectrometry , ion , ion trap , fucosylation , photochemistry , glycoprotein , chromatography , organic chemistry , biochemistry , computer science , operating system
Permethylated acidic and neutral N‐glycans representing different types of glycan structures, such as linear and branched sialylated structures, high‐mannose type and fucosylated complex type, were photodissociated with 157 nm vacuum ultraviolet light in a linear ion trap. Cross‐ring fragments corresponding to high‐energy fragmentation pathways were observed in abundance for all studied structures. Some product ions appear diagnostic for a linkage of sialic acid residues and the glycan antenna to which these residues are attached. A conclusive assignment of the fucosylation site of the studied glycan structure has been facilitated through measurement of cross‐ring fragmentation resulting from photodissociation. Copyright © 2007 John Wiley & Sons, Ltd.