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Determination of hydrogen, carbon and oxygen isotope ratios of ethanol in aqueous solution at millimole levels
Author(s) -
Yamada Keita,
Yoshida Naohiro,
Calderone Giovanni,
Guillou Claude
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2977
Subject(s) - chemistry , aqueous solution , oxygen , hydrogen , ethanol , carbon fibers , isotopes of carbon , kinetic isotope effect , hydrogen isotope , oxygen 18 , inorganic chemistry , radiochemistry , nuclear chemistry , organic chemistry , deuterium , total organic carbon , physics , quantum mechanics , materials science , composite number , composite material
Three stable isotope ratios, D/H, 13 C/ 12 C and 18 O/ 16 O, are measurable in ethanol, an important organic compound that is used as a material for food and beverages, fuel and chemical feedstock, and as a substance related to metabolism. We developed a simple and rapid method of measurement of three isotope ratios of ethanol in aqueous solution at millimole levels using gas chromatography‐high‐temperature conversion or combustion‐isotope ratio mass spectrometry (GC‐TC/C‐IRMS) combined with solid‐phase microextraction (SPME). Using this method, the δ value for ethanol was determined in 30 min for δ D and δ 13 C, and in 75 min for δ 18 O with precisions of ±9‰, ±0.3‰ and ±0.7‰, respectively, for δ D, δ 13 C, and δ 18 O. An advantage of this process is that it requires no distillation for ethanol purification. The method is useful for small quantities of analyte with low ethanol concentrations, which is expected for environmental and metabolic studies. Copyright © 2007 John Wiley & Sons, Ltd.