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Multistage electrospray ionization mass spectrometric analyses of sulfur‐containing iridoid glucosides in Paederia scandens
Author(s) -
Zhou Yan,
Zou Xu,
Liu Xin,
Peng Shulin,
Ding Lisheng
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2965
Subject(s) - chemistry , electrospray ionization , fragmentation (computing) , mass spectrometry , tandem mass spectrometry , electrospray , chromatography , ion trap , ion , analytical chemistry (journal) , organic chemistry , computer science , operating system
Abstract Paederia scandens is a traditional Chinese herbal medicine that possesses important bioactive sulfur‐containing iridoid glucosides. This study reports the investigation of the fragmentation behavior of four sulfur‐containing iridoid glucosides isolated from P. scandens by ion trap electrospray ionization tandem mass spectrometry (ESI‐MS n ). Both multistage electrospray ionization (ESI‐MS n ) experiments in an ion trap instrument and high‐resolution and accurate mass measurement in an ESI‐Q‐TOF mass spectrometer in positive mode were used to elucidate the main fragmentation pathways of these compounds. These experiments yielded essentially the same product ions in both ion trap and Q‐TOF instruments, and their fragmentation patterns were found useful for their characterization, which were applied to elucidate a trace compound in the extracts of P. scandens by on‐line LC/ESI‐MS n . Major and diagnostic product ions have been identified and their origins are proposed. Copyright © 2007 John Wiley & Sons, Ltd.