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Do b ‐ions occur from vibrational excitation upon H‐desorption in electron capture dissociation?
Author(s) -
Haselmann Kim F.,
Schmidt Marcel
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2932
Subject(s) - chemistry , dissociation (chemistry) , ion , fragmentation (computing) , collision induced dissociation , excitation , electron ionization , hydrogen , electron capture dissociation , ammonia , desorption , electron capture , spectral line , photochemistry , analytical chemistry (journal) , mass spectrometry , tandem mass spectrometry , ionization , fourier transform ion cyclotron resonance , adsorption , organic chemistry , chromatography , physics , engineering , operating system , astronomy , computer science , electrical engineering
Electron capture dissociation (ECD) has recently been shown in some cases to produce abundant N‐terminal b ‐ion peptide fragments. These product ions are usually only observed when activation occurs via vibrational excitation as in collision‐induced dissociation (CID). Here, we show that occurrence of b ‐ions in the ECD spectra of synthetic peptides are correlated with low gas‐phase basicity and that the observed b ‐ion fragments are N‐terminal products. Furthermore, all ECD spectra containing b ‐ions also had abundant losses of hydrogen and ammonia from the charge‐reduced species. Copyright © 2007 John Wiley & Sons, Ltd.