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Use of a variably sensitive selected reaction monitoring method to extend the linear dynamic range for quantitative high‐performance liquid chromatography/tandem mass spectrometry
Author(s) -
Fries Harvey E.,
Aiello Mauro,
Evans Christopher A.
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2856
Subject(s) - chemistry , selected reaction monitoring , dynamic range , mass spectrometry , tandem , range (aeronautics) , triple quadrupole mass spectrometer , chromatography , linear range , throughput , analytical chemistry (journal) , tandem mass spectrometry , liquid chromatography–mass spectrometry , detection limit , computer science , telecommunications , materials science , composite material , computer vision , wireless
A method has been devised with the capacity to extend the linear dynamic range of a triple quadrupole mass spectrometer operated in the selected reaction monitoring (SRM) mode of analysis. This extended range experiment can be realized by simultaneously acquiring variably sensitive data, via collision energy adjustment, for the same precursor‐to‐product ion transition within a single SRM method. While this method can be applied universally to many different study types without any detrimental effect to the analysis or throughput, it was applied herein to acquire and quantify, within a single analysis, the concentrations of GSK‐A in a multiple‐dose rodent study, that previously required a dilution scheme. Using this methodology, the linear dynamic range of GSK‐A was increased over traditional methods by nearly two orders of magnitude, from 2.00–10 000 ng/mL to 0.500–100 000 ng/mL. Copyright © 2007 John Wiley & Sons, Ltd.