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Recommended criteria for the mass spectrometric identification of target peptides and proteins (<8 kDa) in sports drug testing
Author(s) -
Thevis Mario,
Loo Joseph A.,
Loo Rachel R. Ogorzalek,
Schänzer Wilhelm
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2848
Subject(s) - chemistry , analyte , chromatography , mass spectrometry , peptide , sample preparation , drug , identification (biology) , combinatorial chemistry , biochemistry , pharmacology , medicine , botany , biology
Mass spectrometry has become an invaluable tool for the identification of prohibited peptide hormones and proteins in doping control analysis. Regulatory authorities have established criteria for identifying banned drugs in doping control specimens, but these criteria do not address the specific issues for high molecular weight protein drugs such as molecular weight determination of multiply charged molecules, analysis of chemically or enzymatically derived degradation products, identification of amino acid sequence tags, etc. Technical considerations such as sample preparation methods (e.g. immunoaffinity purification), resulting analytes (e.g. intact compounds vs. chemically or enzymatically derived peptides), ionization modes, analyzer resolution, and the information provided by respective techniques are discussed in light of sports drug testing requirements using typical application examples. Copyright © 2007 John Wiley & Sons, Ltd.