Premium
Mass spectrometry and scanning electron microscopy study of silicone tunneled dialysis catheter integrity after an exposure of 15 days to 60% ethanol solution
Author(s) -
Guenu Sophie,
Heng AnneElisabeth,
Charbonné Françoise,
Galmier MarieJosèphe,
Charlès Franck,
Deteix Patrice,
Souweine Bertrand,
Lartigue Claire
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2837
Subject(s) - chemistry , silicone , chromatography , catheter , mass spectrometry , ethanol , desorption , scanning electron microscope , surgery , adsorption , biochemistry , organic chemistry , medicine , physics , quantum mechanics
Anti‐infectious lock is an emerging therapeutic option for preventing and/or controlling catheter‐associated infection. Ethanol has widespread bactericidal activity, limited side effects, and low risk of inducing antimicrobial resistance. However, concerns have been raised about ethanol‐induced catheter structural degradation. In this study, silicone catheters were immersed at 37°C in three different solvents: 0.9% sodium chloride, 60% ethanol, and 95% ethanol for 4 h, 15 days and 15 days after a first storage of 4 h. Scanning electron microscopy (magnification 1000–20 000 times) of the inner surface of the catheter revealed no damage to the lumen surfaces of catheters immersed in 95% ethanol for 15 days compared with the reference catheter. Gas chromatography/mass spectrometry (GC/MS) and matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) analysis of the storage solutions revealed a significant release of polydimethylsiloxanes having a number of dimethylsiloxane units lower than 30 in the 95% ethanol solution and a structure highly consistent with a cyclic structure. Most release occurred within the first 4 h of exposure. In contrast, there was no difference in the small amounts of silicone released in 0.9% sodium chloride as reference and 60% ethanol solution, whatever the exposure time. These results should allow the development of clinical trials to assess the efficacy of the 60% ethanol lock technique in preventing or controlling the infectious complications of silicone dialysis catheters. Copyright © 2006 John Wiley & Sons, Ltd.